New datasets: Mouse Single Cell FFPE Demonstration (v1 Chemistry) The complementary nucleotide in the RNA target must be an A." * Updated probe design guidance to include "The 25th nucleotide of the probe (3' most nucleotide of the LHS probe) must be a T. Version of the genome reference used for analysis if a new genome reference is created. * Updated the probe set reference csv section to include additional guidance on modifying the header to match the name and * Updated the URL for registering an instrument and updated the Firmware Update Procedure.Ĭustom Probe Design for Visium Spatial Gene Expression and Chromium Single Cell Gene Expression Flex (CG000621, Rev B) * Added description of unrestricted outbound access and added Connectivity details for more accuracy.Ĭhromium X Series (X/iX) Quick Reference Cards (CG000471, Rev B) * This update describes updated firmware features and bug fixes.Ĭhromium X Series: Network Connectivity Guidelines (CG000508, Rev B) More details to the Firmware Update Procedure, and added examples of Error Screens.Ĭhromium X Series Firmware Release Note (CG000547, Rev B) Option lofs, updated Connectivity section to describe user interface icons, updated information about outbound access, added * Updates to this User Guide include updated first-time use procedure, added export procedure for support packages to the menu Once genes are selected, customers will work with 10x Genomics to finalize details of the panel.Ĭhromium X Series (X/iX) User Guide with Readiness Test (CG000396, Rev E) 10x Genomics recommends the use of relevant single cell RNA-seq data to identify such marker genes and to estimate Interested in selecting markers for specific subpopulations of cells, or genes expressed under specific conditions (e.g.,ĭisease). Pre-designed Xenium panels are designed to cover all major cell types found in a tissue. * This NEW Technical Note provides specific guidance for selecting genes to include on Xenium add-on panels. Xenium Add-on Panel Design (CG000643, Rev A) Hosts and port, local connectivity, and storage requirements. * Updates to this Technical Note include additional technical details regarding outbound connectivity, detailed whitelisting of Xenium Analyzer: Network Connectivity Guidelines (CG000645, Rev B) As supply constraints have resolved, we are removing our recommendation of this alternative product, and asking Connect customers return to using only the preferred Eppendorf twin.tec® 150 µl PCR Plate, 96-well, full-skirted plate on their systems. These plates were originally validated as an alternative full-skirted plate to use on the Chromium Connect system, during a time when sourcing of the preferred Eppendorf plate was constrained. Notice about Amplifyt™ plates with Chromium Connect: 10x Genomics has recently removed their recommendation of: Amplifyt™ 96-Well PCR Plates, Full-Skirted, Clear, PN #1149K05, also known as PN 485096 (available from Thomas Scientific) or Catalog No. Update instructions and software update package are available at our Chromium Connect support website. An in-depth resource detailing features in this current software upgrade is available online here. Bug fixes and visual interface improvementsįor a complete list of what’s new in our updated Chromium Connect software, please see the release note.New remote support activation/deactivation screen.New popup alert when selecting “Expert” instruction level.New assay setup loading from previously canceled run during loading phase-allows users to selectively decrease the number of samples in a Chromium Connect run.New Software Update: We have improved the Chromium Connect system and encourage you to update today! To access the latest quarterly update and support improvements on Chromium Connect, we are asking that all users update their Chromium Connect instrument to the latest software version (v2022.12.30).
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