![]() Lewis, B.P., Burge, C.B., and Bartel, D.P. (2003) Expression profiling reveals off-target gene regulation by RNAi. (2007) MicroRNA targeting specificity in mammals: determinants beyond seed pairing. (2005) The widespread impact of mammalian microRNAs on mRNA repression and evolution. (2003) Asymmetry in the assembly of the RNAi enzyme complex. (2003) Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening. (2007) Insights into effective RNAi gained from large-scale siRNA validation screening. (2007) Comparison of approaches for rational siRNA design leading to a new efficient and transparent method. ![]() (2006) Genome-wide functional analysis of human cell-cycle regulators. (2005) Design of a genome-wide siRNA library using an artificial neural network. SiRNAs are screened for multiple sequence motifs known to result in an interferon response. This increases siRNA potency, as an siRNA spanning a SNP will vary in its effectiveness. The RefSNP database is used to exclude siRNAs that span single nucleotide polymorphisms (SNPs). Asymmetry produces highly functional siRNAs and reduces the risk of off-target effects caused by the incorrect strand entering RISC.Īnalysis uses intelligently weighted, multi-parameter searches for matches of the seed region of the siRNA antisense strand with the 3' untranslated region of unintended mRNA targets (see text for further explanation). This enables the antisense strand, which is less tightly bound at its 5' end, to enter RISC, while the sense strand is degraded. SiRNAs are designed with unequal stabilities of the base pairs at the 5' ends. Genomewide analysis enabled development of siRNA design improvements that minimize off-target effects.Ĭurrent data from NCBI databases ensure accurate design. A large number of druggable genome siRNAs have been proven to provide at least 70% knockdown during this project.Īnalysis uses a proprietary tool and an up-to-date, nonredundant sequence database. The design process was reinforced and improved by data from this project, in which QIAGEN scientists proved the effectiveness of thousands of siRNAs. The world's largest siRNA validation project Up Next: Learn more about oversized cover plates.SiRNA design uses the BioPredsi neural-network, which is based on an extremely large RNAi data set. Standard Quote: Just need a quote for regular items stocked on our site? Simply add items to your cart and print. (Larger quantities reduce the per plate price.) Please contact us for a quote. Custom sizes start at $100 per job, or more if a specialized punch shape is needed. (Please contact us first then attach it to our reply.) Please be sure it is a straight-on photo (so the box or plate is square in the photo) so we can more easily take measurements to ensure you get the correct size.Ĭustom Switch Plates Starting at $100 - Get a Quote on a Customized Size: Need a switchplate size that we don't carry to accommodate a construction project? If you need a specialized size, we have the capability to stamp those, too. If possible, please send us a photo of your old wall plate or of the devices in the box. ![]() ![]() Still Can't Find A Size? Contact Us! We can help you find a fast, affordable solution. Recessed Outlet Covers: If you are trying to mount a flat screen TV or computer monitor flush on the wall, use recessed outlet covers. ![]()
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